EBAE II: Electric Boogaloo

Posted on May 11, 2012

2


Woot! This past week’s meeting, EBAE II, was a great success! It was a great discussion and relaying of some useful tips and tricks. Many thanks to Koji Takahashi and Forrest Collman for their contributions. And thanks to the SUPD Postdoc Initiative Fund for funds to cover dinner.

Here are the annotated slides from Tuesday’s discussion.

I have also the audio from this session (which is quite poor, sorry! my mic was dying) here and the previous session’s audio is here.

  • NB: I’ve got grand visions of transcribing these discussions in my free time, but you see, I don’t have any free time. If you’d like to assist me in this task, I’d greatly appreciate the help!

Lastly, if you have ideas for other topics to be discussed in future sessions, please do let me know! This is all about sharing useful nuggets of information that are otherwise hard to find/learn.

 


Addendum:

Some folks from the Deisseroth lab have provided recipes for NMDG slicing solutions. They provided some useful details about them:

Here are receipes for the NMDG cutting solution and modified HEPES ACSF for holding slices until recording. The procedure is to perfuse and slice with NMDG, leave the slices in NMDG for 5-10 min (apparently the optimal time to leave your slices in NMDG is somewhat brain area dependent…)  at 33deg, then do 3 x 10 min washes with the HEPES ACSF before starting recording. The NMDG solution is a huge pain in the ass. There’s a bunch of stuff that needs to get made up fresh each day and apparently the NMDG only has a useful shelf life of ~3wks, so you have to keep reordering. 

Advertisements
Posted in: general