Browsing All Posts published on »October, 2011«

Some recipes and more!

October 26, 2011

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I’ve attached some recipes for ACSF and internal solutions here. Thanks to Suzanne and Dervis for sending me their recipes! I’m slowly, but sure working through the transcript of our session (and a huge thanks to Astra Bryant for her assistance!). Hope to have it up soon. In the meantime, I’ll pose a question: What was one of […]

discuss…

October 21, 2011

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Some folks after the meeting suggested we post questions every week or so; things concerning your process or technique. Some suggestions were: What are aspects of your prep you obsess about?  What is the most common source of trouble on your rig? (noise, flow issues, pipets) What was one of the most ‘valuable’ learning experiences […]

Slides from today’s discussion

October 20, 2011

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Here they are, the EBAE slides. Print. Copy. Enjoy.   An amended version incorporating some of the discussed techniques/approaches will be posted in the coming days..   .

linked papers from 10.20.11

October 20, 2011

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Here are links to the papers that were mentioned in the discussion on 10.20.11 General: Ephys for Dummies Sakmann & Neher: Single Channel Recordings Slicing issues: Aitken, P. G., et al (1995). Preparative methods for brain slices: a discussion. Journal of Neuroscience Methods 59, 139–149. Lipton, P., et al. (1995). Making the best of brain […]

The patch starts here

October 18, 2011

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This is a site dedicated to the pain that is endemic to neuroscience research. A few postdocs and grad students at Stanford University were commiserating about the vagaries and seemingly voodoo-esque nature of patch-clamp slice physiology. We reasoned that if we pooled our knowledge (and did a bit of background reading), we could shed light […]